pcdna3 rluc polires fluc (Addgene inc)
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pcdna3 rluc polires fluc
Pcdna3 Rluc Polires Fluc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+rluc+polires+fluc/pcDNA3+RLUC+POLIRES+FLUC+(Plasmid+%2345642)/bio_rxiv__2025__09__05__674545-218-11-12
Average 93 stars, based on 49 article reviews
Pcdna3 Rluc Polires Fluc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+rluc+polires+fluc/pcDNA3+RLUC+POLIRES+FLUC+(Plasmid+%2345642)/bio_rxiv__2025__09__05__674545-218-11-12
Average 93 stars, based on 49 article reviews
pcdna3 rluc polires fluc - by Bioz Stars,
2026-10
93/100 stars
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Luciferase:Article Title: A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescence. Article Snippet: SuperScript VILO cDNA synthesis kit (Invitrogen) was used for cDNA synthesis and qRT-PCRwas performed (primer sequences provided in Table S5). .. Translation fidelity luciferase assays The dual luciferase assay for measuring stop codon readthrough or amino acid misincorporation was performed as previously described.87 Briefly, pGL3-Renilla-Ter-firefly or pGL3-Renilla-noTer-firefly plasmids were generated by cloning a Renilla luciferase ORF with or without a TAA termination codon upstream of a firefly luciferase ORF in pGL3-Control (Promega). pcDNA3-RenillaFLAG-firefly was generated by replacing the IRES sequence in Article Title: α-Ketoglutarate dictates AMPK protein synthesis for energy sensing in human cancers Article Snippet: .. For luciferase reporter vectors, PRKAA1 5′UTR or scramble 5′UTR DNA sequences were synthesized and cloned into Article Title: α-Ketoglutarate dictates AMPK protein synthesis for energy sensing in human cancers. Article Snippet: .. For luciferase reporter vectors, PRKAA1 5′UTR or scramble 5′UTR DNA sequences were synthesized and cloned into Article Title: Article Snippet: .. Generated:Article Title: A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescence. Article Snippet: SuperScript VILO cDNA synthesis kit (Invitrogen) was used for cDNA synthesis and qRT-PCRwas performed (primer sequences provided in Table S5). .. Translation fidelity luciferase assays The dual luciferase assay for measuring stop codon readthrough or amino acid misincorporation was performed as previously described.87 Briefly, pGL3-Renilla-Ter-firefly or pGL3-Renilla-noTer-firefly plasmids were generated by cloning a Renilla luciferase ORF with or without a TAA termination codon upstream of a firefly luciferase ORF in pGL3-Control (Promega). pcDNA3-RenillaFLAG-firefly was generated by replacing the IRES sequence in Cloning:Article Title: A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescence. Article Snippet: SuperScript VILO cDNA synthesis kit (Invitrogen) was used for cDNA synthesis and qRT-PCRwas performed (primer sequences provided in Table S5). .. Translation fidelity luciferase assays The dual luciferase assay for measuring stop codon readthrough or amino acid misincorporation was performed as previously described.87 Briefly, pGL3-Renilla-Ter-firefly or pGL3-Renilla-noTer-firefly plasmids were generated by cloning a Renilla luciferase ORF with or without a TAA termination codon upstream of a firefly luciferase ORF in pGL3-Control (Promega). pcDNA3-RenillaFLAG-firefly was generated by replacing the IRES sequence in Sequencing:Article Title: A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescence. Article Snippet: SuperScript VILO cDNA synthesis kit (Invitrogen) was used for cDNA synthesis and qRT-PCRwas performed (primer sequences provided in Table S5). .. Translation fidelity luciferase assays The dual luciferase assay for measuring stop codon readthrough or amino acid misincorporation was performed as previously described.87 Briefly, pGL3-Renilla-Ter-firefly or pGL3-Renilla-noTer-firefly plasmids were generated by cloning a Renilla luciferase ORF with or without a TAA termination codon upstream of a firefly luciferase ORF in pGL3-Control (Promega). pcDNA3-RenillaFLAG-firefly was generated by replacing the IRES sequence in Synthesized:Article Title: α-Ketoglutarate dictates AMPK protein synthesis for energy sensing in human cancers Article Snippet: .. For luciferase reporter vectors, PRKAA1 5′UTR or scramble 5′UTR DNA sequences were synthesized and cloned into Article Title: α-Ketoglutarate dictates AMPK protein synthesis for energy sensing in human cancers. Article Snippet: .. For luciferase reporter vectors, PRKAA1 5′UTR or scramble 5′UTR DNA sequences were synthesized and cloned into Clone Assay:Article Title: α-Ketoglutarate dictates AMPK protein synthesis for energy sensing in human cancers Article Snippet: .. For luciferase reporter vectors, PRKAA1 5′UTR or scramble 5′UTR DNA sequences were synthesized and cloned into Article Title: Coronavirus M proteins disperse the trans-Golgi network and inhibit anterograde protein trafficking in the secretory pathway Article Snippet: To generate the ERSE-luciferase construct pairs of phosphorylated and annealed oligos for 2 ERSE sequences separated by a spacer, were cloned into pGL4.26 (Promega) using Bgl II and Acc 65I. .. To generate the CMV-Renilla construct the Renilla gene was cloned from Article Title: α-Ketoglutarate dictates AMPK protein synthesis for energy sensing in human cancers. Article Snippet: .. For luciferase reporter vectors, PRKAA1 5′UTR or scramble 5′UTR DNA sequences were synthesized and cloned into Construct:Article Title: Coronavirus M proteins disperse the trans-Golgi network and inhibit anterograde protein trafficking in the secretory pathway Article Snippet: To generate the ERSE-luciferase construct pairs of phosphorylated and annealed oligos for 2 ERSE sequences separated by a spacer, were cloned into pGL4.26 (Promega) using Bgl II and Acc 65I. .. To generate the CMV-Renilla construct the Renilla gene was cloned from Article Title: Article Snippet: .. Plasmid Preparation:Article Title: α-Ketoglutarate dictates AMPK protein synthesis for energy sensing in human cancers Article Snippet: .. A Article Title: α-Ketoglutarate dictates AMPK protein synthesis for energy sensing in human cancers. Article Snippet: .. A Transfection:Article Title: α-Ketoglutarate dictates AMPK protein synthesis for energy sensing in human cancers Article Snippet: .. A Article Title: α-Ketoglutarate dictates AMPK protein synthesis for energy sensing in human cancers. Article Snippet: .. A other:Article Title: A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescence. Article Snippet: After 24 hours, cells were transfected with 2.5 mg of reporter plasmids using Lipofectamine 3000 transfection reagent (Invitrogen). |
